Transcriptome-wide Analysis of Exosome Targets
نویسندگان
چکیده
منابع مشابه
Transcriptome-wide Analysis of Exosome Targets
The exosome plays major roles in RNA processing and surveillance but the in vivo target range and substrate acquisition mechanisms remain unclear. Here we apply in vivo RNA crosslinking (CRAC) to the nucleases (Rrp44, Rrp6), two structural subunits (Rrp41, Csl4) and a cofactor (Trf4) of the yeast exosome. Analysis of wild-type Rrp44 and catalytic mutants showed that both the CUT and SUT classes...
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The RNA exosome complex functions in both the accurate processing and rapid degradation of many classes of RNA. Functional and structural analyses indicate that RNA can either be threaded through the central channel of the exosome or more directly access the active sites of the ribonucleases Rrp44 and Rrp6, but it was unclear how many substrates follow each pathway in vivo. We used CRAC (UV cro...
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ژورنال
عنوان ژورنال: Molecular Cell
سال: 2012
ISSN: 1097-2765
DOI: 10.1016/j.molcel.2012.08.013